Membrane Biology — DU B. Sc. (Hons) Biochemistry Previous Year Question Papers

4. (a) Discuss the structure and function of Nat-Rt (c) Discuss the structure and mechanism of 5. (a) Diagrammatically elaborate on me RBC cytoskeleton and briefly mention the role of different cytoskeletal proteins. (b) Discuss the key events that led to an understanding of the structure of the biological membrane. (c) Briefly describe a technique to explain the transverse movement of lipids in a membrane. 6. Write short notes on the following: (i) Lipid Rafts (ii) Homeoviscous adaptation (iii) Membrane fusion (v) FRAP technique (3×5) 2. There are 6 questions. 1. (a) Define the following terms : (ii) Fence and gate model (iv) Lipid anchored proteins (vi) Facilitated diffusion (vii) Single Particle Tracking (viii) Critical micelle concentration (b) Give contributions of the following scientists : (i) Bert Sakmann (ii) S. J. Singer and G. L. Nicolson 2. (a) Give explanations for the following : (i) RBCs are good models for studying biological membranes. (i) Integral membrane proteins require detergents for their solubilization. (iii) MDR pumps are also called molecular vacuum cleaners. (iv) Critical micellar concentrations of ionic detergents decrease with the addition of electrolytes. (v) Hydropathy plots are useful for predicting membrane-spanning segments of a peptide. (vi) Cholesterol plays an important role in the structure and function of the biological membranes. (b) Discuss the roies of enzymes in catalyzing trans-bilayer translocations of phospholipids. (12.3) 3. Differentiate between the following : (i) Peripheral proteins and Integral membrane proteins (iii) Ion channels and Ionophores (iv) P-Type and F-Type ATPases (v) Primary Active and Secondary Active transport