Metabolism of Carbohydrates — DU B. Sc. (Hons) Biochemistry Previous Year Question Papers

There are 6 questions. Attempt four questions in all. (A) Define the following terms: (b) Amphibolic pathway (c) Lactose intolerance (d) Substrate level phosphorylation (f) Pasteur effect. (B) Give reasons for the following : (a) Arsenate inhibits ATP generation in Glycolysis (b) Galactosemia is associated with cataract. (c) Hemolysis occurs in patients with Glucose-6-P-dehydrogenase deficiency. 9,6 2. Give the biochemical role of the following : (a) TPP in Pyruvate dehydrogenase reaction (b) Biotin in Pyruvate carboxylase reaction (c) Malate dehydrogenase enzyme in Gluconeogenesis (d) Aldolase B in fructose metabolism (e) Glucokinase in liver. 5x3 3. (a) Explain how the key regulatory enzyme phospho-fructokinase-I is regulated. (b) Give the diagrammatic representation of oxidative decarboxylation of pyruvate to generate Acetyl CoA by Pyruvate dehydrogenase complex. (c) Gluconeogenesis is not reversal of glycolysis. Justify the statement. 3×5 4. Differentiate between the following : (a) Lactic acid fermentation and Alcohol fermentation (b) Branching enzyme and Debranching enzyme (A) Give the following biochemical reactions in the TCA cycle : (a) Oxidative decarboxylation reactions (b) Dehydrogenase reactions (c) Hydration and dehydration reactions. (B) Explain how do the following molecules impact carbohydrate metabolism (b) Sodium fluoride Give short notes on the following : 5×3 (a) Anaplerotic reactions (c) Metabolic adaptation during starvation (d) Cori cycle (e) McArdle disease.