Basic Bacteriology — DU B. Sc. (Hons) Microbiology Previous Year Question Papers

1. " (a) Give one example of the following (any ten) : 1x10=10 (i) Spiral shaped bacterium (iž) Complex medium (üi) "Bacterium having proteinaceous capsule (iv) Gram negative bacterium (u) Aerobic bacterium (vi) Bud producing bacterium (vili) Bacterium lacking cell wall (ix) Bacterium/group having membrane bound nucleoid (x) Largest bacterium : (xi) Bacterium showing grape like arrangement. (b) Discuss any two physical factors which affect bacterial growth. 5 (c) What is the significance of high surface area to volume ratio in bacterial cells ? 3 2. (a) Write short notes on (any three) : 4x3=12 (iii) Streak. plate technique (io) Bacterial flagellar arrangements (b) Give composition and function of the following inclusion bodies (any two): 3×2=6 (22z) Gas vacuole. 3. (a) Differentiate between the following (any three) : 4x3=12 (i) Protoplasts and spheroplasts (ii). Capsule and slime layer (iii) Selective and differential medium (b) Draw a well labelled diagram of Gram-positive flagellum. (c) How pencillin inhibit bacterial cell wall synthesis ? 2 (c[) Briefly explain the foHowing terms (any ffaree) : (jL)) Batch culture. (a) Draw a well labeued diagram of Gram-negative cell will. (c) Define generation time and growth rate. Derive the mathematical expression. for generation time. 6 .(c!) twhat is the role ofMicrobial culture collection centers? Give name of any fr{;a such centers. 5. (a) Explain any orae method for counting viable bacterial cells. 6 (c) Define plasmid and explain any fzuo types with examples. 4 (a) Comment on bacterial fimbriae. 3 6. (cz) . What is teichoic acid? Give its functions. 4 (a) Discuss viable but non-culturable bacteria. 4 (c) Draw well labelled diagram of bacterial endospore. How is it able to survive harsh conditions? ` 7 (cZ) Comment on fragmentation of bacteria.