Chem-DSE-8: Biomolecules-II — DU B. Sc. Life Science (Chemistry) Previous Year Question Papers

All questions have equal marks. (c) Explain temperature and pH effects on enzyme activity with bell-shaped curve graphs. (d) Distinguish catabolism (exergonic) and anabolism (endergonic) with energy flow diagram. (4,4,4, 3) Q.4 (a) Compare the role of NAD* and FAD as electron carriers in cellular respiration. Mention the type of reactions they usually participate in and where they are regenerated (b) Discuss the Edman's method in primary structure determination of Peptides. (c) Describe the principle of the ninhydrin test and write the general reaction showing the formation of the characteristic colour. (d) Define the caloric value of food and state the standard caloric contents (kcal/g) of carbohydrates, proteins, and fats. (4,4,4,3) Q.5 (a) What do you mean by Active site of Enzymes? What are the main characteristics of the active site? (b) Discuss the Merrifield synthesis of polypeptides. Write the advantages associated with the solid-phase peptide synthesis? (c) Fill in the blanks with appropriate word: (i) High-energy phosphate bond compound is known as .... (i)........... is a process of anaerobic production of ethanol from glucose. (ii) An organic cofactor like FAD (flavin adenine dinucleotide) is called a... (iv) At pH 6, basic amino acids migrate toward the........ in electrophoresis. (d) Explain the fate of pyruvate under anaerobic conditions in muscle cells and yeast, respectively? (4,4,4,3) Q.6. (a) Write short note on the following with suitable reactions (any three): (i) Glycolysis- overview and significance (ii) Denaturation of proteins (iii) Gabriel Phthalimide synthesis (iv) Structure-activity relationship (SAR) of drug molecules (b) Describe the role of FAD as an organic cofactor (coenzyme) in metabolic reactions.?