Genetic Engineering Technologies & Applications — DU B.Sc. (Hons.) Botany Previous Year Question Papers

4. Attempt Four questions in all. Q1. a) Write the use of the following enzymes 5 marks i) Restriction Endonuclease ii) DNA Ligase: ili) Tag Polymerase iv) Kinase v) Reverse Transciptase b) State True or False: 5 marks i) YACs are derived from bacterial chromosomes. ii) PEG-mediated transformation is used only in animals. iii) A genomic library contains all the DNA sequences of an organism. iv) Automated sequencing uses capillary electrophoresis. v) Recombinant insulin is produced using rDNA technology. c) Match the following: 5 marks i) Gene cloning PCR ii) Kary Mullis Electric pulse iii) Northern Blotting [DNA Technology iv) Paul Berg Multiple copies of gene v) Electroporation RNA Q2. Differentiate between (any three): 3 X 5=15 marks i) RT- PCR and Q-PCR ii) Selectable marker genes and reporter genes ili) YAC and BAC iv) Genomic Library and cDNA Library Q3. Write short notes (any three): 3 X 5= 15 marks i) Human Genome Project ii) Particle bombardment ill) Screening of DNA Library iv) Sanger sequencing method Q4. a) Describe plasmids as vectors in genetic engineering. 5 marks b) Explain in details Agrobacterium mediated transformation. 10 marks Q5. a) Discuss the gel based electrophoresis (semi- automated) sequencing method. Explain how it is different from Capillary based electrophoresis (automated) sequencing method. 8 marks b) Discuss applications of rDNA technology in human health, including insulin, interferons, and 7 marks vaccines. Q6. a) Describe the construction of a cDNA library. 7 marks b) Explain the methods used for screening and identification of desired clones from a DNA library. 8 marks