Plant Biotechnology — DU B.Sc. (Hons.) Botany Previous Year Question Papers

5. All parts of a question should be answered together. Ql.a) Fill in the blanks: 5 marks 1) Flavr savr Tomato was developed by ... biotechnology company. il) The genetically modified organism for bioremediation developed by A.M. Chakraborty is known as ili) is known as the father of plant tissue culture. iv) The bacterial cells ready for the uptake of foreign DNA are known as v) Cellular totipotency was discovered for the first time by.......... b) Match the following: 5 marks i) Golden Rice a) Stanley Cohen and Paul Berg il) Reporter gene b) Hind II ili) Restriction Endonucleases c) Sipra Guha and S.C. Maheshwari iv) Androgenesis d) Ingko Potrykus v) First recombinant DNA molecule e) GUS 5 marks c) Define the following: i) Totipotency ii) Superbug iii) Luciferase iv) Triploids v) Microprojectile bombardment Q2. Write short notes on (any five): i Micropropagation ii) Artificial seeds ill) Selectable marker genes iv) Agrobacterium mediated transformation v) Moondust carnations vi) Cybrids Q3. Differentiate between (any three): i) Organogenesis and somatic embryogeneisis ii) Type I and type II restriction endonuclease ill) Vaccines and edible vaccines iv) Electroporation and microinjection Q4. a) Explain cloning and selection of transformants. 8 marks 7 marks b) Describe the role of plasmids as vectors in cloning. Q5. a) Describe and discuss five major applications of recombinant DNA technology. 10 marks b) Why are nutrient mediums important for plant tissue culture? Discuss the role of vitamins and 5 marks plant growth regulators in nutrient medium. Q6. a) Trace the historical development of plant biotechnology, highlighting key milestones and 7 marks paradigm shifts. b) Discuss the applications of transgenic plants in enhancing crop productivity. 8 marks