Biosensors and Nanotechnology (INDSE8A) — DU B.Sc. (Hons) Instrumentation Previous Year Question Papers

(a) Name different Nanomaterials used in detection of biomolecules along with their (6) unique properties. Also discuss how different nanomaterials are revolutionizing the biosensor industry. (b) Why do ID nanowires exhibit different optical properties compared to 3D bulk (6) (c) How does the increase in surface area affect the melting point and hardness of a (6) metal nanomaterial? 5. (a) What is meant by immobilization? Explain the techniques entrapment and cross (6) linking used for immobilization. (b) Differentiate between biological and electronic amplification in a biosensor. (6) Explain how signal transduction is achieved in electrochemical biosensors. Explain the role of nano sensors used in drug delivery system and point-of-care (6) 6. (a) What is meant by Signal to Noise (SNR) ratio in biosensor design? What is its (6) importance? Outline one chemical and one electrical method used to minimize noise. (b) Compare DNA-based biosensors and immunosensors in terms of their (6) biorecognition principles. Provide one application for each and explain why selectivity is vital in their design. (c) Explain the process of Ball Milling method for synthesis of nanomaterials. List (6) some of its advantages and disadvantages. 7. (a) What are the primary dimensional classifications of nanostructures? Explain with (6) suitable examples. What is the primary bottom-up methods used for the synthesis of quantum dots? (6) (C) A 5 MHz AT-cut quartz crystal biosensor is used to detect the binding of a (6) specific antibody. The active electrode area of the crystal is 0.5 cm?. After the antibody binds to the surface, a stable frequency shift (Af) of -45Hz is recorded Calculate the total mass change (Am) obtained after binding. (Density of quartz=2.648 g/cm?, 3, Shear modulus of quartz: 2.947×10" g cm' s3)