Animal Biotechnology — DU B.Sc. (Hons.) Zoology Previous Year Question Papers

6. Write short notes pn any three of the following: (a) Restriction enzymes (b) Chimeric DNA (c) Knockout mice (d) Cloning Vectors : Animal Biotechnology NEP-UGCF Time .. 2 Hours (b) Attempt four questions including Question 1. (a) Define (any three.): (.i) Insertional inactivation (ii) In vivo gene therapy (iv) Expression vector (b) Distinguish between (any three): 2x3-6 (i) Blunt and Cohesive ends : (iii) Dolly and Polly (iv) M13 phage and lambda phage (c) Expand the following: 0.5×4=2 (d) Give the important contributions of the following scientists: 1x4=4 (i) D. T. Burke and G. F. Carle (ii) H. W. Boyer and S. N. Cohen (iv) Werner Arber 2. (a) Describe the method of construction of CDNA library with the help of suitable diagram 8 (b) Explain Colony hybridization method for screening of genomic libraries. 7 3. (a) Describe microinjection method for production of transgenic animals. 8 (b) Explain application of transgenic animals in production of pharmaceuticals. 7 4. (a) Describe the procedure of production of (b) Explain molecular diagnostic techniques for detection of Sickle cell anemia. 7 5. (a) Explain the CRISPR/Cas9 system and its applications in gene editing 8 (b) Explain SCID. Describe method used for its gene therapy 7