Medical Diagnostics — DU Skill Enhancement Courses Previous Year Question Papers

SEC Course Proposed by Department of Biochemistry and Daulat Ram College Track : Laboratory Techniques Series 1. CREDIT DISTRIBUTION, ELIGIBILITY AND PRE-REQUISITES OF THE COURSE Credit distribution of the Coursetitle course Eligibility Pre- requisite &Code criteria of the course Lecture Tutorial Practical 2 0 2 XII pass None 2. Learning Objectives This course aims to train the students in skills required in biochemical, hematological and immune- diagnostics through the use of various procedures, methods and equipment for the purpose of disease diagnosis. The students are skill trained to develop the knowledge, technical and critical thinking skills essential to safely and reliable collection and analysis of patient samples. The will be skill trained to work in diagnostics labs in hospitals, research/ pathology/ Laboratories and Pharma industry. 3. Learning Outcomes After the completion of the course the students will be able to Process and store bio-samples following Biosafety practices. Perform various Biochemistry assays. Perform Haematological analysis & diagnosis of diseases. Perform Immunodiagnostic assays. Record data and analyse report. 4. Main Course Structure Unit 1 - Laboratory safety and standards 4 Hours Biosafety Practices, Sample collection, processing, labelling, preservation and record keeping. Bio-waste disposal techniques Unit 2- Hematological Investigations 16 hours RBC count, Erythrocyte sedimentation rate, packed cell volume Total Leukocyte count, Differential Leukocyte count, Absolute Eosinophil count Hemorrhagic disorders: Clotting time Blood group determination Unit 3- Biochemical Investigations 28 hours Biochemical markers for disease. Serum, sputum and urine analysis. Assays of salivary markers of acute myocardial infarction: CK-MB/ cTn (cardiac markers) Diabetes markers Estimation of Serum electrolytes: Na, K, Ca, P Estimation of Serum bilirubin, direct and indirect, Serum Transaminases : SGPT/SGOT ( Liver Function Test) Serum Hormone assays: Thyroid, TSH (ELISA) (endocrine Function) Analysis of Urine for abnormal constituents: Proteins, sugar, ketones Unit 4 -Immuno Diagnostics 8 Hours Rapid Antigen assay. RT-PCR test Unit 5-- Visit to a Diagnostic lab 4 Hours Automation and real lab scenario experience 5. Teaching Methodology/Activities in the classroom Introduction to basic concepts and the principles of the practicals to be performed with power point presentations. Students made aware of safety protocols and which nave to be strictly followed. Hands on training of the advance instruments to be used and the lab practicals to be performed 6. Assessment Pattern for each Unit/practical. Unit 1: 10 marks Student will be assessed by viva- voce, quiz, written assessment and on participation in various activities and practicals in class on the following topics: 2. Collection and storage of samples. 3. Bio waste disposal techniques Unit 2: 20 marks