Biotechnology — DU B. Sc. (Hons) Biochemistry Previous Year Question Papers

2. Attempt 4 questions in all. 1A State the statements whether true or false and justify your statement: i) Adenovirus based gene transfer in mammalian cells is used to achieve stable transfection. ii) Pseudomonas putida is used for bioremediation of oil spills in the oceans. iv) Recombinant insulin expressed in E. coli must undergo post-translational modifications identical to human insulin. All genetically modified plants are considered completely safe for human consumption without testing. vi) Recombinant enzymes cannot withstand extreme pH or temperature conditions in industries. B. Write the name of the diseases) associated with the deficiency of following proteins: i) Factor VIII 2A. Write different physical methods for transfer of a foreign gene into mammalian cells. B. Explain the steps involved in producing recombinant human insulin using E. coli as host system. C. Explain the steps involved in the preparation of cheese. 3A. Decibe the citinent stages involved in sewage water treatment and explain the B. What is bt cotton, and how does its genetic modification help in pest resistance? C. Explain gene therapy. Describe its application in treating inherited disease using gene therapy. 4A. Define single cell protein. Discuss the advantages and limitations of using SCP as an alternative protein source for humans and animals. B. Describe the process of Somatic Cell Nuclear Transfer (SCNT) taking a appropriate example. C. Describe the role and applications of antisense RA in plant gene regulation. (5, 5, 5) 5A. Explain the steps involved in the preparation of beer. B. Define Molecular Pharming. Explain how recombinant proteins are produced using transgenic plants. C. Discuss how animal biotechnology techniques contribute to improving livestock productivity. 6. Write short notes on the following: