Microbial Quality Control and Testing — DU B. Sc. (Hons) Microbiology Previous Year Question Papers

1. Write short notes on the following (any three) : 3x4=12 (a) Biosensor based detection (b) Resazurin Reduction Test (d) Clot on boiling test. = 2: Explain the following terms (any six): 6x2=12 (a) Personal Protective Equipment (b) Limulus Lysate test (c) Microbial limits of sterility testing (d) Selective media* (e) Multiplex PCR ) Biosafety cabinets (g) Differential media. - (a) Explain the principle and application of-membrane filtration method in , bioburden testing. 4 (b). Describe the principle and method of any one technique used for antimicrobial susceptibility testing. 4 (e) What are the BIS and ESSAI, and how do they contribute to food safety? 4- (a) Compare microscopic and culture based methods for microbial detection. 6 (b) Mention any two ISO certifications relevant to food or pharmaceutical industries. 2 (c) Explain the procedure and principle of the Most Probable Number (MPN) 5. (a) How can nucleic acid probes be used for detection of microorganisms ? 4 (b) Provide examples of microorganisms handled at each biosafety level (c) Discuss the key criteria for ensuring drinking water potability. What role do quality control strains play in this process ? 4 (a). Explain the key principles of Good Microbiological Laboratory Practices (GMLP). How do they contribute to lab safety and quality? 6 (b) Why is it important to validate sterilization methods and culture media in microbiological testing ? 2 (e) Describe the phenol coefficient test and its role in determining disinfectant efficacy. 4