Basic Laboratory and Field Skills in Plant Biology — DU B.Sc. (Hons.) Botany Previous Year Question Papers

Q1. Answer the following questions 15 (a) Fill in the blanks (any five) (5x1=5) (i) In a confocal microscope, the out-of-focus light is eliminated by passing emitted light ) The malality of 100ml or a solution taken from a 2M solution will be (iii) For a random sample of 16 students, the average CGPA 8, and the sample standard deviation is S = 4. The standard error of the sample is is an example of secondary database. (v) According to Beer-Lambert law, the absorbance of a solution is directly proportional to the of the absorbing species and the path length. (vi) Logio1000 is equal to b). Match the column (5x1=5) i. Cell size A ii. Biohazard B Hemocytometer ili. Cell number C Environmental Hazard D Micrometer c) Define (any five) (5x1=5) (i) Lux meter (iii) Preservative (iv) Stock Solution (v) Scientific Notations Q2. Differentiate between the following (any five) (5X3=15 (i) Precision and Accuracy (i) Bar Graph and Histogram (iii) Median and Mode (iv) Conductivity meter and pf meter (V) LB Media and YEB Media (vi) Fluorescence microscope and Confocal microscope Q3. Write short notes on following (any three) (3X5=15) (i) Grades of laboratory chemicals based on purity (ii) Sampling Methods (iii) Agarose Gel Electrophoresis (iv) Steps for Herbarium preparation Q4. Answer the following questions: 15 b) From the following data recorded on the height of the plants of varieties G-65 and PS- 16 of Mungbean; find out which variety is more consistent using coefficient of variation? (8) Varieties Plant height (in cm) QS. Answer the following questions: 15 a) Discuss the various bacterial culture techniques with its applications. (7) b) Determine the number of bacteria present in original bacterial culture (10ml) using the provided serial dilution image. 10mL Buffer Buffer Buffer Bacterial culture 9mL 9mL 9mL Volume Plated 1mL 1mL 1mL Pour Plate Count 261 26 (c) Calculate the molarity of a sulfuric acid solution (molecular weight = 98 g/mol) prepared by dissolving 196 g of the acid in water to make a final volume of 500 ml. Additionally, if a young researcher mistakenly diluted 2 L of a 6 M sulfuric acid solution by adding 4 L of water, what will be the new molarity of the resulting solution? (4)