Genomics, Proteomics and Bioinformatics — DU B.Sc. (Hons.) Botany Previous Year Question Papers

AND BIOINFORMATICS Maxinun Marks: 60 d) All parts of the question must be answered together. e) Draw diagrams wherever required. (a) State whether the following statements are true or false (any five): i. ii. iii. iv. v. vi. Metagenomics studies cultured microorganisms only. Histone acetylation generally represses gene expression. Non-coding DNA has no biological function. KEGG is a protein structure database. 2-D gel electrophoresis separates proteins by charge and size. Uniprot is a database for protein sequence and functional information. a) Expand the following (any five): 1x5=5 I. 11. The technique used to identify DNA-protein interactions is The study of genetic material recovered directly from environmental samples is known as Ill. In two-dimensional gel electrophoresis (2-DE), isoelectric focusing separates proteins based on their IV. The database contains three-dimensional structural data of biomolecules. Highly repetitive DNA sequences present in the genome are referred to as 2. Write short notes (any three): 3x5-15 i. Explain the principles and applications of genome editing. iii. Describe the principles and steps involved in protein-ricroarray analysis. 3. Differentiate between (any three): 3x5=15 i. Coding DNA and non-coding DNA. ii. Two-dimensional (2D) gel electrophoresis and SDS-PAGE. iii. Primary and secondary biological databases iv. Structural genomics and functional genomics. 4. (a) Describe the technique of nano liquid chromatography-\tandem mass spectrometry (nLC-MS/MS) and critically evaluate its applications in modem proteomics. (8 marks) (b) Explain the principle and applications of MALDI-TOF mass spectrometry. (7 marks) 5. (a) The NCBI is a widely used bioinformatics resource. Discuss the major tools and databases hosted by NCBI. (8 marks) (b) Define sequence alignment. Explain the concepts of local, global, and multiple sequence alignment with the help of suitable diagrams. (7 marks)