Animal Cell Biotechnology — DU B.Sc. (Hons.) Zoology Previous Year Question Papers

(b) Explain transgenic animal technology. Explain the difference in generation of animal Dolly and 5. (a) Describe DNA fingerprinting. (8) (b) Write about construction of libraries. Explain how genomic library construction is different from cDNA library preparation. (7) 6. (a) Discuss Biosafety. (8) (b) Discuss Fermentation in detail. (7) 7. Write short notes on any three of the following: (a) Stem cells (b) Hybridoma technology (c) Sanger sequencing (d) Expression vectors Draw well-labelled diagrams wherever necessary. (a) Define the following : (i) Phagemid (ii) Cryopreservation of cell culture (iii) Chromatography (iv) Biological containment (v) cDNA library (b) Differentiate between the following : (4×2=8) (i) BAC and YAC (ii) Northern blotting and Western blotting (c) State whether the following statements are True or False : (4×1=4) (i) Biotechnology is applied only in the medical field. (ii) Electroporation is the method used to introduce DNA into animal cells. (iii) Batch fermentation is a continuous process with constant nutrient addition. (iv) Hybridoma technology is used for producing monoclonal antibodies. (d) Fill in the blanks : (4×1=4) (i) The enzyme that cuts DNA at specific recognition sites is called . (ii) The technique used to amplify DNA in vitro is called . (iii) The treatment of diseases by correcting defective genes is called . (iv) Cells taken directly from tissues and grown in culture for the first time are called culture. (e) Match the following : (4×1=4) Column A Column B (i) Trypsin a. Maintains pH. (ii) Fetal Bovine Serum b. Sterile working area. (iii) CO2 incubator c. Cell detachment enzyme. (iv) Laminar air flow d. Nutrient supplement. 2. (a) Explain the scope of biotechnology. Discuss its application. (7) (b) Describe different types of cloning vectors used in gene transfer. Explain bacteriophages and plasmid in detail. (8) 3. (a) Explain principles of animal cell culture. Discuss types of culture. (8) (b) Describe different types of culture media used in animal cell culture. Explain the role of serum and growth factors. (7) 4. (a) What is electrophoresis. Explain the types of electrophoresis based on gel composition. (7)