Principles of Molecular Biology-II — DU B. Sc. (Hons) Microbiology Previous Year Question Papers

(i) Phillip A Sharp and Richard J Roberts (ii) Jacob and Monod 5. (a) Describe the mechanisms of regulation of the /rp operon in E. co/i.. (7) (b) Provide a brief overview of how ribosomal RNA (rRNA) is processed from its precursor molecule in eukaryotes. (5) (c) Identify and explain any two mechanisms that ensure the fidelity of translation in prokaryotes. 6. Write short note on (any three) : (6.x3=18) (i) Genetic control of sporulation in Bczcz.//#s (ii) Charging of tRNA (iv) Positive and negative transcriptional control in 1. Define the following (any nine) : (2x9=18) (i) MAT locus (iv) Split genes (vi) Non sense codons (vili) Cotranscriptional loading (ix) RNA interference (x) Translational coupling 2. (a) Differentiate between the following (any three) : (i) Group I and Group II introns (iv) Histone acetylation and Histone methylation (b) Write the significance of the following : (any six) 3. (a) Discuss the steps in the termination of translation in prokaryotes. (6) (b) Briefly describe the process of trans-splicing with a relevant biological example. (6) • (c) Discuss the steps involved in the capping of eukaryotic mRNA and write its significance. (a) Define long non-coding RNAs and explain their role in regulating gene expression with an example. (b) Expand and give significance of the following (any five) : (2×5=10) (c) Describe the contribution of the following scientists (any one) : (2)