Genomics, Proteomics and Metabolomics — DU B.Sc. (Hons.) Botany Previous Year Question Papers

Q1. a) Match the following: (5X1=5) (i) Illumina Secondary antibodies (il) Emulsion PCR DNA Library (iti) Heterochromatin Mobile phase (iv) Silica Beads (v) Alkaline phosphatase Repetitive sequences b) Explain in brief-any five of the following: : (5 X 2= 10) i. Importance of in third generation sequencing in genomic research. Clone-by-clone sequencing yields a more accurate final genome assembly compared to whole genome shotgun sequencing. iv. Role of different protein structures in functional aspect of proteins. V. HPLC is used as analytical technique in metabolomics. vi. In an ELISA, it is crucial to have a washing step after each incubation period. Q2. Differentiate between (any five): (5 x 3= 15) (i) Western blotting and ELISA (ii Primary metabolites and secondary metabolites (iii) Primary structure and Secondary structure of proteins (iv) Column chromatography and lon-exchange chromatography Q3. Write short notes on any three: (3 X5 = 15) (i) Model organisms (il) Rice genome project (iv) SDS-PAGE (V) Proteomics and its application Q4. (a) What is chromatography? What are the different types of chromatographic techniques used in proteomics? (ii) Explain the different types of post translational modification with examples? (7) Q5. (i) Explain the procedure of classical sequencing methods used for sequencing of organism with larger genome. (8) (ii) What is Next generation sequencing? Enumerate the different types of NGS platforms?