Recombinant DNA Technology and Proteomics — DU B.Sc. (Hons.) Botany Previous Year Question Papers

(b) Describe various methods of introducing DNA into host cells (bacterial and plant systems) and compare their efficiencies. 5. (a) Explain different types of cloning vectors (plasmids, bacteriophage vectors, artificial chromosomes) with suitable examples. 8 (b) Explain PCR and its applications in generation of target DNA for cloning. 7 6. (a) A recombinant clone needs to be identified from a genomic library. Explain how DNA hybridization and immunological methods can be used for its screening and confirmation. 7 (b) Discuss applications of recombinant DNA technology and proteomics in medicine with suitable example. 8 Technology and (b) Attempt four questions in all. Question (d) Illustrate your answer wherever possible. 1. (a) Define the following (any five): (i) Liposomes (ii) Expression vector (iv) Competent cells (v) Reporter gene (b) Expand (any five): 1×5=5 (c) Match the following (any five): 1×5=5 (ii) Reverse transcriptase b. Separation based on pland molecular weight (iii) Antibiotic resistance c. First widely used artificial plasmid cloning vector (iv) Ligase d. Same recognition sequence, different origin (vi) pBR322 f. Plasmid with a phage origin (vii) pBluescriptIl 8. Molecular glue 2. Differentiate between the following (any three): (i) Linkers and adapters (iii) Genomic library and DNA library (iv) 2D Gel Electrophoresis and SDS-PAGE (v) Insertion and Replacement Vectors 3. Write short notes on the following (any three): (i) Antisense strategy in tomatoes (ii) Alpha-complementation (ill) Western Blotting (iv) Affinity chromatography (v) Mass spectrometry 4. (a) Describe restriction endonucleases and explain their role in recombinant DNA technology. 8