Genome Organization and Function — DU B. Sc. (Hons) Biomedical Previous Year Question Papers

Attempt five questions in all. Attempt the sub-parts of the questions together. Draw illustrations or diagrams, wherever required. 1. (a) Define (any four) : 4x1=4 (i) Chromodomain proteins * * (ii) Nucleosome positioning (iii) CRISPR-Cas system (0) mRNA' decay. ' ** 1 (b) Justify the following statements as True or False (any five) : 5x2=10 (i) Methylation of Lysine at the 9th position of H3 (H3K9) is associated with "Transcriptional Activation (i) miRNAs and siRNAs both regulate gene expression by targeting mRNAs. : (u) Unitary pseudogenes originate from functional genes with the accumulation of degenerative mutations. (vi) Repetitive DNA sequences are absent in the human genome. (e) Expand the following acronyms (any four) : 4x1=4 2. Distinguish between (any three): 3×6=18 (i) Activators and co-activators - (i) Gene families and Superfamilies (iii) Chromodomain proteins and bromodomain proteins (iv) Ubiquitin-proteasome pathway and lysosomal degradation. 3. Comment on the following (any ffo7iee)' : (Z) riRNA Decay (a.i) Gene Families and Sup.erfumilies (Zz.i) Translational repressor (Zz;) Insulator prevents enhancer-promoter interactions. 4. (q) Explain various mechanisms by which bacterial cells can regulate peRNA_ expre s sion. 6 (a). Discuss riboswitehes and. describe how they can serve` as potential targets E=-for antibiotics. 6 (c) Explain .`how enhancers and sil.encers regulate gene expression at a distance. Why are enhancers ithportant in tissue-specific gene. 5. (a[) Define insulators and boundary elements. 4 (a) Explain how insulators prevent enhancer-promoter interactions. 8 (c) Dis'cuss the significance of insulators in genome organisation. 6 6. (ai) Define sRNAs and their role. (c) How do sRNAs .improve bacterial adaptability ?