Concepts in Biotechnology — DU B. Sc. (Hons) Biomedical Previous Year Question Papers

Attempt five questions in all. Give illustrations and examples wherever required. Subparts of the questions should be attempted together. Q2. (b) Write short notes on (any 3) (3x3=9 marks) (i) Alkaline lysis method of Plasmid isolation (ii) Non-viral vectors in gene therapy (iv) Real Time PCR Q 3. (a) Give role/Significance of the Following: (6x2= 12 marks) (i) Calcium Chloride in transformation (iv) Lac Z' Gene (v) Magnesium in PCR (b) What are the key features of an expression vector. What are the challenges associated with cloning a heterologous gene in E coli. (6 marks) Q 4. (a) Give schematic represenattion of: (3x4=12 marks) (1) Western Blotting (ii) Multiplex PCR Q4. (b) What are the ethical concerns in case of genetically modified orgamisms and their use for commercial purposes? Enumerate with examples. (6 marks) Q5. (a) What is DNA profiling? Explain its application with example. (6 marks) Q5. (b) Explain in detail the technique of DNA profiling with the help of a diagram. Q5. (c) What are the factors to keep in mind while designing primers for a PCR reaction? 06. (a) Compare and contrast the features of a plasmid vector to those of a phage lambda vector. Give examples. (6 marks) Q6. (b) Give the principle of any one DNA sequencing method. (6 marks) Q6. (c) What are lambda replacement and lambda insertion vectors. Give examples