Advanced Molecular Biology and Genetic Engineering — DU B. Sc. (Hons) Biomedical Previous Year Question Papers

(b) Describ-e any one method to study protein-protein interactions. (8) 4. (a) How does chromatin organization regulate gene expression? (7) (b) Describe the production of transgenic plants or animals with one example. (8) 5. (a) A virus integrates its DNA into the host genome using site-specific recombination. (i) Explain site-specific recombination. (ii) What role do enzymes like integrase play? (iii) Compare homologous vs site-specific recombination. (iv) How can viral integration affect host gene expression? (10) (b) Discuss why biosafety regulations are necessary for GMOs. (5) Attempt four questions in all. 4. Give illustrations and examples wherever required 5. Subparts of the questions should be attempted together. 1. (a) Briefly explain the following : (Any 5) (5xl=5) (i) Genetic code degeneracy (ii) Reverse transcriptase (iii) Point mutation (b) Justify the following statements as true or false : (i) DNA exists only in the double helix (duplex) form in living cells. (ii) Nucleosomes are formed by DNA wrapping around histone proteins. (iii) A gene cloned into a vector will always be expressed in the host cell. (iv) Genetic engineering has no ethical or legal concerns. (v) Fusion proteins can help in purification of racombinant proteins. (vi) Histidine tags are commonly used to purify fusion proteins. 2. (a) Differentiate between the following : (Any 3) (i) Plasmid vectors Vs 7i bacteriophage vectors (iii) Hoogsteen base pairing Vs Watson-Crick base pairing (iv) Positive gene regulation Vs Negative gene regulation (b) What are Type Il restriction enzymes? (3) 3. (a) Explain how transposons contribute to antibiotic resistance. (7)