Medical Biotechnology — DU B. Sc. (Hons) Biomedical Previous Year Question Papers

AND debrau, 1911201 3. Subparts of the questions should be attempted together. 4. Please provide illustration wherever required for more clarity. (a) Provide one word for the following statements: (i) Process of increasing the number of copies of small, circular DNA molecules replicating independently of chromosomal DNA inside the cell. (ii) A technique used to introduce DNA, RNA, or other molecules into cells by applying an electrical field to create temporary pores in the cell membrane. (iii) Type of cloning vector designed to replicate in multiple host organisms, typically both in prokaryotic (like E. coli) and eukaryotic cells. (iv) The part of a 2 replacement vector that is removed during insertion of new DNA. (v) A labeled nucleic acid molecule that can be used to identify complementary or homologous molecules through the formation of stable base-paired hybrids. (b) Justify the following statements as true or false (any six) : (6x1.5=9) (i) Gene cloning is preferred over PCR for obtaining an amplified gene sample. (1I) In the genetic map of the 2 DNA molecule, genes with similar functions are randomly distributed throughout the genome. (111) Chemical lysis method of DNA isolation typically requires one agent to target the cell wall and another to disrupt the cell membrane. (iv) Phenomenon of host-controlled restriction was a key observation that prompted further research into restriction endonucleases. (v) Treatment with a cold salt solution, particularly calcium chloride, has been shown to enhance the efficiency of DNA transformation in E. coli. (vi) Random priming uses DNA polymerase I for synthesizing new DNA due to its lack , of nuclease activity. (vii) Strong promoters are a boon for effective (b) I recombinant protein expression. (c) Identify the scientists associated with the following achievements. (4) (i) The discovery of Restriction Enzymes (ii) Development of first approved gene therapy treatment (iv) Discovery of green fluorescent protein (a) Differentiate between the following (any three) : cl (i) Single-stranded and Double-stranded in vitro packaging (ii) Nick translation and Random priming (iv) Cloning Vector vs. Expression Vector (b) Indicate the significance / role of the following (i) X-Gal in a-complementation assay (ii) SDS in genomic DNA isolation (iv) Mg2+ in PCR reaction (v) Cofactors in DNA Ligases (Vi) Antibiotics used in cloning experiments 3. (a) Indicate the maximum DNA insert size possible and the host that can be used with the following cloning vectors (any five): (5x2=10) (vi) Viral vectors (b) What are the challenges associated with expressing recombinant protein in E. coli. How are these addressed? (4) (c) What are the various method used to screen recombinant colonies after transformation? (4) 4. (a) Schematically represent the following processes (any four): (4x4=16) (i) DE3-based recombinant protein expression